Gene encoding enzymes that function in the biosynthesis of arginine and proline were manually annotated. Bioinformatics tools including BLAST, KEGG, MetaCyc, T-Coffee, Weblogo, and CDD were used to examine gene products predicted by annotation to be enzymes 1.2.1.88, 2.1.3.3, 2.72.11, 3.5.1.2.The enzyme commission (ec) numbers 1.2.1.88(L-Glutamyl P); 2.1.3.3 (ornithine carbamoyltransferase); 2.7.2.11(Glutamate-5-kinase); 3.5.1.2 (Glutaminase) and their enzymatic function were analyzed. These gene products function as enzymes in the arginine and proline metabolic pathway of Brachybacterium faecium.
